A functional assay of protein C in human plasma
نویسندگان
چکیده
منابع مشابه
A functional assay for protein C in human plasma.
We describe a functional assay for protein C in human plasma samples based on the ability of activated protein C to prolong the kaolin-cephalin activated partial thromboplastin time of normal plasma. Protein C is separated from its inhibitor by elution of a barium citrate precipitate, and activated by incubation with human alpha-thrombin for one hour. Thrombin is then inhibited by antithrombin ...
متن کاملIdentification of functional endothelial protein C receptor in human plasma.
The endothelial cell protein C receptor (EPCR) binds protein C and facilitates activation by the thrombin-thrombomodulin complex. EPCR also binds activated protein C (APC) and inhibits APC anticoagulant activity. In this study, we detected a soluble form of EPCR in normal human plasma. Plasma EPCR appears to be approximately 43, 000 D, and circulates at approximately 100 ng/ml (98.4+/-27.8 ng/m...
متن کاملNewand Rapid Functional Assay for Cl Inhibitor in Human Plasma
c i’ inhibitor (Ci-INH) and a2-macroglobulin (a2M) account for over 90% of the inactivation of purified plasma kallikrein by normal human plasma. The rate of kallikrein inactivation is also dependent on the presence of high molecular weight kininogen (HMWK). which forms a reversible complex with kallikrein protecting the active site of the enzyme against inhibitors. By selectively mactivating a...
متن کاملA High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma
A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium dicl...
متن کاملDetermination of Protein Concentration Using Bradford Microplate Protein Quantification Assay
Background: Bradford protein assay is popular due to its ease of performance and relative sensitivity. Many researchers and laboratories in Iran use standard assay of Bradford by cuvette. No commercial kit was available for Bradford microplate assay in Iran. Meanwhile, imported Bradford commercial kits are very expensive and have a long delivery time in Iran. Till now no study or document on Br...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Blood
سال: 1984
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v63.3.671.671